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Fluorescence In Situ Hybridization
Fluorescence in situ hybridization (FISH) has long been an essential standard method in molecular pathology. In this technique, fluorochrome-labeled probes bind directly—in situ—to complementary target sequences in the genome within cytology preparations or tissue sections, without the need for nucleic acid extraction or amplification. FISH is a rapid and cost-effective approach that enables reliable analysis even in very small samples, including biopsies or cytologic specimens with only a few dozen tumor cells. Evaluation of FISH results requires a fluorescence microscope.
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