Chromogenic In Situ Hybridization
Chromogenic in situ hybridization, CISH, was developed as an alternative to FISH. Instead of direct fluorescent labeling of the probe, various haptens - digoxigenin, biotin and dinitrophenyl - are used, which are detected in a multi-stage process via the formation of chromogenic precipitates.
These preparations can be analyzed with a normal light microscope, which is available on every pathologist´s desk. In addition, the morphological context can be assessed more easily than with FISH, and the preparations have a longer shelf life.
The CISH product group is divided into two product lines:
ZytoDot®
Overnight hybridization and detection the following day.
ZytoFast®
Hybridization in one to two hours and detection on the same day. All products for pathogen diagnostics fall into this category.
