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Fluorescence In Situ Hybridization
Fluorescence in situ hybridization, FISH, has long been one of the indispensable standard methods in molecular pathology: fluorochrome-labeled probes bind directly in the tissue section - in situ - to the complementary target sequence in the genome; extraction and amplification of nucleic acids is not carried out. FISH is a fast and cost-effective method that also enables reliable analysis of very small biopsies with only a few dozen tumor cells. A fluorescence microscope is required to analyze FISH preparations.
